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Key Documents

03285

Sigma-Aldrich

Cell Counting Kit

sufficient for 500 tests

Synonym(s):

Fluorescent Cell Counter

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About This Item

UNSPSC Code:
12352116
eCl@ss:
32161000
NACRES:
NA.32

form

liquid

Quality Level

usage

sufficient for 500 tests

storage temp.

−20°C

General description

Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.

Application

Cell Counting Kit or FCCK is used for the detection of live cells by fluorometric method.

Features and Benefits

Cell Counting Kit has the following advantages:

  • It doesn’t require any radioisotopes to function.
  • No solubilization procedure is needed.
  • Results are highly reproducible and accurate.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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