06966

Atto 655-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPCE)

• Synonym(s): Biotin-Atto 655
• UNSPSC Code: 12352116
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• Assay: ≥90% (HPCE)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• transmittance: 254 nm; 655 nm
• solubility: DMF: soluble; DMSO: soluble; acetonitrile: soluble
• fluorescence: λ_ex 655 nm; λ_em 680 nm in 0.1 M phosphate pH 7.0
• λ: in ethanol (with 0.1% trifluoroacetic acid: 655 nm ± 3 nm)
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

Application

Atto 655 conjugated to biotin is useful for binding to proteins (i.e., avidin and streptavidin). The fluorescent properties include excellent thermal and photo-stability, high quantum yield, strong absorption (663 nm) and fluorescence (684 nm), and low triplet formation. It is a zwitterionic dye, carrying a net electrical charge of zero. Electron donors such as guanine and tryptophan can easily quench the fluorescence of this fluorophore. Biotin conjugated with ATTO 655 was applied to assess efficiency of BSA/avidin structures bound to a surface.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves