A0410

Aminoallyl-dUTP sodium salt

≥85%, lyophilized powder

• Synonym(s): 5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP
• Empirical Formula (Hill Notation): C₁₂H₂₀N₃O₁₄P₃ · xNa⁺
• CAS Number: 936327-10-5
• Molecular Weight: 523.22 (free acid basis)
• MDL number: MFCD06200396
• UNSPSC Code: 41106305
• NACRES: NA.51

Properties

• Quality Level: 200
• Assay: ≥85%
• form: lyophilized powder
• shipped in: dry ice
• storage temp.: −70°C
• SMILES string: [P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)\C=C\CN)O)(O[P](=O)(O)O)O.N
• InChI key: YWKTUHBZWBRYHX-TYYBGVCCSA-N

Description

Application

Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.

Aminoallyl-dUTP sodium salt has been used:

• to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
• for the generation of chromatin immunoprecipitation (ChIP) probes
• in the reverse transcription of RNA for the synthesis of fluorescent labelled probes

Biochem/physiol Actions

Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves