A9728

Monoclonal Anti-ATP Synthase β antibody produced in mouse

1 mg/mL, clone 4.3E8.D10, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD03455681
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4.3E8.D10, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 50 kDa
• species reactivity: human, mouse, rat
• concentration: 1 mg/mL
• technique(s): immunoprecipitation (IP): suitable; indirect immunofluorescence: suitable; western blot: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ATP5B(506) ; mouse ... Atp5b(11947) ; rat ... Atp5b(171374)

Description

General description

ATP synthase is a highly conserved transmembrane protein that can catalyse the reversible synthesis of ATP from ADP and phosphate.

Detects the β subunit of ATP synthase and can be used as a mitochondrial marker.

Immunogen

intact rat mitochondria.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Mouse Monoclonal Anti-ATP Synthase beta antibody can be used for western blot, indirect immunofluorescence and immunoprecipitation assays.

Pancreatic islet cells isolated from wither Wistar ratos or nondiabetic organ donors were fixed in 4% paraformaldhyde and permeabilized with 0.3% Triton-X for immunocytochemistry using monoclonal mouse anti- beta ATP synthase at a 1:1000 dilution.

Physical form

Solution in phosphate buffered saline containing 1.0 mg/mL bovine serum albumin and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable