B3148

Anti-Goat/Sheep IgG−Biotin antibody, Mouse monoclonal

clone GT-34, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Biotin antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: biotin conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: secondary antibodies
• clone: GT-34, monoclonal
• form: buffered aqueous solution
• species reactivity: sheep, goat, bovine
• should not react with: rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human
• technique(s): direct ELISA: 1:5,000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Goat IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

The product binds to all goat/sheep IgG1

Application

Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse has been used in:

• enzyme linked immuno sorbent assay (ELISA)
• immunoblotting
• dot blotting
• immunohistochemistry
• immunocytochemistry

Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse may be used for ELISA at a minimum working dilution of 1:5000. It was used at a working dilution of 1:1000 to visualize collagen type IV in mouse digit sections.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable