B3679

BCIP^®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

• Synonym(s): 5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system
• UNSPSC Code: 12352204
• NACRES: NA.83

Properties

• Product Name: BCIP^®/NBT-Purple Liquid Substrate System for Membranes, ready to use alkaline phosphatase substrate
• Quality Level: 100
• form: liquid
• storage temp.: 2-8°C

Description

Application

BCIP^®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase

• for the detection of secondary antibodies by immunoblot
• in ELISPOT assay
• in dot blot analysis
• in western blot to visualize protein bands on nitrocellulose membrane

Biochem/physiol Actions

5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.

Caution

Stable at least one year at 2-8 °C.

Physical form

Ready-to-use.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable