B9139

Biotin-dextran

mol wt 10,000, Lysine-fixable

• MDL number: MFCD00130657
• UNSPSC Code: 12352201
• eCl@ss: 34058011
• NACRES: NA.25

Properties

• conjugate: biotin conjugate
• Quality Level: 200
• form: powder
• mol wt: 10,000
• extent of labeling: ~2 mol per mol
• color: white
• solubility: H₂O: soluble 50 mg/mL, clear, colorless to faintly yellow
• storage temp.: 2-8°C

Description

Application

Biotin-dextran is used in tissue staining to prepare neurons for detection by avidin-conjugated detection systems. It is taken up by neuronal processes and transported bi-directionally. It infiltrates axon boutons in the anterograde direction and dendritic processes in the retrograde direction. Staining can be observed in fixed and sectioned tissue from two days to two weeks after biotin-dextran is injected into the brain or spinal cord. It can also be applied to cut nerve tracts. Biotinylated neurons are detected by either light or electron microscopy following incubation with avidin-horseradish peroxidase conjugate and the electron-dense substrate 3,3′-diaminobenzidine (DAB).

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves