C1235

Cholesterol Oxidase microbial

recombinant, lyophilized powder, ≥10 units/mg protein

• Synonym(s): Cholesterol: oxygen oxidoreductase
• CAS Number: 9028-76-6
• EC Number: 1.1.3.6 (BRENDA, IUBMB)
• EC Number: 232-842-1
• MDL number: MFCD00130783
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥10 units/mg protein
• mol wt: 55 kDa
• solubility: 50 mM potassium phosphate buffer, pH 7.0: soluble
• storage temp.: −20°C

Description

Application

Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H₂O₂. Optimum pH of the enzyme is 7.0. Hg²⁺, Ag⁺, ionic detergents inhibit the enzyme activity.

Unit Definition

One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 °C and pH 7.0 in a peroxidase linked system.

Preparation Note

Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves