C1744

Phosphoenolpyruvate carboxylase microbial

≥5.0 unit/mg solid

• Synonym(s): PEPC
• CAS Number: 9067-77-0
• MDL number: MFCD00131862
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥5.0 unit/mg solid
• mol wt: ~390 kDa
• storage temp.: −20°C

Description

Application

Phosphoenolpyruvate carboxylase (PEPC) is an enzyme useful for enzymatic determination of carbon dioxide when coupled with malate dehydrogenase in clinical analysis. PEPC is also used to study carbon assimilation, post-translational regulation and allosteric regulation in various plants .

Biochem/physiol Actions

Phosphoenolpyruvate carboxylase (PEPC) catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate and inorganic phosphate . In CAM and C⁴ plants, PEPC catalyzes the photosynthetic assimilation of CO2 into an organic acid. PEPC is activated by glucose-6-phosphate and is inhibited by malate and aspartate. PEPC comprises about 0.5-2% of the soluble protein in alfalfa and soybean nodules .

Unit Definition

One unit causes the oxidation of one micromole of NADH per minute at pH 8.0 at 30°C.

Physical form

White amorphous lyophilized powder containing BSA and sugar alchohols as stabilizers.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable