C4187

Cyanoborohydride Coupling Buffer

• Synonym(s): Coupling buffer
• UNSPSC Code: 12161700
• NACRES: NA.56

Properties

• form: liquid
• Quality Level: 200
• reaction suitability: reagent type: reductant
• technique(s): affinity chromatography: suitable
• application(s): life science and biopharma
• storage temp.: 2-8°C

Description

Application

A ready-to-use reagent used to couple amine ligands to aldehyde functional groups. The coupling buffer reaction is a reductive amination of the intermediate Schiff′s base to a stable C−N bond.

Cyanoborohydride Coupling Buffer has been used:

• in coupling reactions between amines and glutaraldehyde
• to reduce hydrazone bond to a stable hydrazide bond
• as a component in oligonucleotide reaction mixture for coverslips functionalization

Cyanoborohydride Coupling Buffer is used in affinity chromatography, protein chromatography, activated/functionalized matrices and synthetic reagents. Cyanoborohydride has been used to inform a safe and effective gene-transfer system targeting hepatocytes as well as to develop a method for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas.

Biochem/physiol Actions

Cyanoborohydride Coupling Buffer is a reagent suitable for reductive amination processes, that contributes to transformation of simple alcohols into more complex amines. It is used in the conversion of Schiff base, by reducing it, to form a secondary amine without affecting aldehyde groups on the support.

Preparation Note

0.02 M sodium phosphate, pH 7.5, containing 0.2 M sodium chloride and 3.0 g/L sodium cyanoborohydride

Safety Information

• Pictograms: GHS07,GHS09
• Signal Word: Warning
• Hazard Statements: H302,H411
• Precautionary Statements: P264 - P270 - P273 - P301 + P312 - P391 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable