C5788

Complement C5a human

recombinant, expressed in E. coli, ~95% (SDS-PAGE), lyophilized powder

• Synonym(s): C5a anaphylatoxin, rC5a
• CAS Number: 80295-54-1
• MDL number: MFCD00130842
• UNSPSC Code: 12352202

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ~95% (SDS-PAGE)
• form: lyophilized powder
• technique(s): activity assay: suitable
• UniProt accession no.: P01031
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... C5(727)

Description

Application

Complement C5a human is an important terminal component of the complement cascade. It is a direct mediator of inflammation, and has been identified as a novel biomarker for pain and inflammation following surgery

Biochem/physiol Actions

A mixture of C5a (~35%) and C5a having an added methionyl residue at the amino terminus (~65%); exhibits biological activities similar to serum-derived C5a. C5a is a (11.2kDa) proteolytic fragment of the C5 α-chain through the action of C5 convertases in the classical and alternative complement pathway (C4b2a4b, C3bBb3b). C5a is an anaphylatoxin. It acts as an inflammatory chemoattractant. C5a stimulation of human neutrophils leads to STAT3 phosphorylation on Ser⁷²⁷. It mediates IL-8 release from bronchial epithelial cells. C5a anaphylatoxin activity on hepatocytes results indirectly from interaction with nonparenchymal cell via prostanoid secretion.

C5a, in addition to being a direct mediator of inflammation, can induce both IL-8 (interleukin-8) synthesis and high levels of IL-8 release from monocytes. This secondary effect serves as an amplification mechanism for inflammation at sites of infection or trauma. C5a exerts its effect through a G-protein coupled receptor, CD88.

Quality

Mol. Wt.: ~8.6 kDa (non-glycosylated, with glutathione attached to cysteine 27).

Other Notes

View more information on the complement pathway at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves