C8118

Chymase human

recombinant, expressed in Pichia pastoris

• Synonym(s): Mast cell protease I
• CAS Number: 97501-92-3
• EC Number: 3.4.21.39 (BRENDA, IUBMB)
• MDL number: MFCD00132173
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in Pichia pastoris
• Quality Level: 300
• form: liquid
• specific activity: ≥40 units/mg protein
• mol wt: ~37 kDa by SDS-PAGE
• concentration: 125-400 μg/mL
• UniProt accession no.: P23946
• application(s): diagnostic assay manufacturing
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CMA1(1215)

Description

Application

Chymase has been implicated in generation of angiotensin II and cleavage of big endothelin. Studies indicate it may be involved in vascular proliferation, myocardial infarction and dermatitis.

Human chymase has been used in a study to assess the effects of Panax notoginseng flower extract on the TGF-β/Smad signal transduction pathway in heart remodeling. Human chymase has also been used in a study to investigate the blood glucose level and survival in streptozotocin-treated human chymase transgenic mice.

Biochem/physiol Actions

The enzyme rapidly converts angiotensin I to angiotensin II. Optimum pH for the enzyme activity is between 7.5 and 9.0. Enzyme activity is inhibited by soybean trypsin inhibitor, phenylmethylsulfonyl fluoride and chymostatin.

Physical properties

Chymase is a cathepsin G-like, S1 serine proteinase found primarily in mast cells. It has a molecular mass of ~30 kDa, however its apparent molecular mass on SDS-PAGE is around 37 kDa.

Unit Definition

One unit hydrolyzes one micromole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7.8 and 25 °C. The assay buffer used to determine the enzyme activity contains 27 mM Tris-HCl, pH 7.8, with 150 mM NaCl and 0.43 mM BTEE.

Physical form

Supplied as a solution in 20 mM Tris, 0.8 M NaCl and 25% glycerol, pH 7.6

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves