CE0050

CelLytic^™ MEM Protein Extraction Kit

• Synonym(s): Membrane protein extraction kit
• UNSPSC Code: 41116134
• NACRES: NA.56

Properties

• technique(s): protein extraction: suitable
• Quality Level: 200
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

CelLytic^™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.

Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.

Application

CelLytic^™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.

Suitability

Sufficient reagents supplied for 80 tests.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• Lysis and Separation Buffer 50 mL
• Wash Buffer for CelLytic MEM 50 mL
• Sodium Chloride, 4M Solution 1.5 mL

Kit Components Also Available Separately

• P8340 Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): 188.6 °F - closed cup
• Flash Point(C): 87 °C - closed cup