Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

CS0780

Sigma-Aldrich

β-N-Acetylglucosaminidase Assay Kit

sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)

Synonym(s):

Beta-NAG Activity Assay Kit

Sign Into View Organizational & Contract Pricing


About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 50 reactions (1 mL)
sufficient for 500 reactions (100 μL)

Quality Level

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... NAGLU(4669)

Application

The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.

Biochem/physiol Actions

N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.

Analysis Note

The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.

Kit Components Only

Product No.
Description

  • Dilution Buffer 8 mL

  • 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg

  • Citrate Buffer Solution, 0.09 M 100 mL

  • p-Nitrophenol Standard Solution, 10 mM 1 mL

  • β-N-Acetylglucosaminidase from Jack beans 1 vial

  • Sodium carbonate 5 g

related product

Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral

Target Organs

Liver,Kidney

Storage Class Code

11 - Combustible Solids


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service