E1782

Anti-EDEM2 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-ER degradation enhancer, mannosidase alpha-like 2
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa
• species reactivity: mouse, rat, human
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: suitable; western blot: 0.5-1.0 μg/mL using whole extracts of human HepG2 and rat NRK cells.
• UniProt accession no.: Q9BV94
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... EDEM2(55741) ; mouse ... Edem2(108687) ; rat ... Edem2(296304)

Description

General description

Three EDEM homologs, EDEM1, EDEM2 and EDEM3 have been identified, which are transcriptionally upregulated upon ER stress by the activated IRE1/Xbp-1 branch. EDEM2 is localized to the ER, mainly as a soluble glycoprotein, interacts with calnexin and lacks mannosidase activity.

Application

Anti-EDEM2 antibody produced in rabbit is suitable for indirect immunofluorescence and immunoblotting at a working concentration of 0.5-1.0μg/mL using whole extracts of human HepG2 and rat NRK cells.

Biochem/physiol Actions

EDEM2 (ER degradation-enhancing α-mannosidase-like protein 2), a stress-regulated mannosidase-like protein, targets misfolded glycoproteins for degradation in an N-glycan dependent manner. Over-expression of EDEM2 accelerates ERAD (ER-associated degradation) by promoting the release of terminally misfolded glycoproteins from the calnexin cycle, without affecting the rate of degradation of non-glycosylated polypeptides or the maturation of model secretory proteins.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves