EDEGLY
Enzymatic Protein Deglycosylation Kit
Synonym(s):
Glycosylation Release Kit
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
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General description
Enzymatic protein deglycosylation kit contains all the enzymes and reagents required for the complete removal of N-linked and simple O-linked carbohydrates from glycoproteins. This kit is employed to cleave the complex Core 2 O-linked carbohydrates including those containing polylactosamine.
Application
Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.
Enzymatic protein deglycosylation kit has been used in:
- protein deglycosylation
- deglycosylation of tissue factor pathway inhibitor (TFPI) α and β
- deglycosylation of native exo-β-(1,3)-glucanase EXG1
Features and Benefits
Enzymatic protein deglycosylation kit has the following features:
- two or more mg of glycoprotein is deglycosylated
- single reaction at neutral pH
- native & denaturing procedures
- no degradation of protein
- O-linked sugars containing polysialic acid are eliminated
- control glycoprotein provided
Kit Components Only
Product No.
Description
- PNGase F 1 mL/vial
- O-Glycosidase 20 μL
- α-2(3,6,8,9)-Neuraminidase 20 μL
- Fetuin Control .5 mg
- 5× Reaction Buffer .2 mL
- Denaturation Solution .1 mL
- Triton™ X-100 .1 mL
- β-1→4-Galactosidase, positionally specific 20 μL
- β-N-Acetylglucosaminidase 20 μL
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Description
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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