F2043
Anti-β-Tubulin−FITC antibody, Mouse monoclonal
clone TUB 2.1, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-β-Tubulin
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250 G
₹7,535.00
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
TUB 2.1, monoclonal
form
buffered aqueous solution
mol wt
antigen 55 kDa
species reactivity
human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster
storage condition
protect from light
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This Item | 86216 | P2443 | 713538 |
|---|---|---|---|
| application(s) detection | application(s) - | application(s) - | application(s) - |
| Quality Level 100 | Quality Level - | Quality Level 200 | Quality Level 100 |
| impurities ≤0.5% water | impurities ≤1.0% water | impurities - | impurities ≤1.0% water |
| mp 56-61 °C | mp 40-50 °C | mp - | mp - |
| hydroxyl value 6‑9 mg KOH/g | hydroxyl value 15‑19 mg | hydroxyl value - | hydroxyl value - |
General description
β-Tubulin belongs to the subfamily of tubulins. It has two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain, which has the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)). TUBB1 (tubulin beta class I) is located on human chromosome 6p21.33.(10)
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.
Specificity
The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.
Immunogen
tubulin from rat brain.
Application
FITC-β tubulin antibody may be used for detection ajnd localization of β-tubulin by direct labeling in a variety of eukaryotic cells ranging from human embryonic kidney cells (HEK293T) to cells of azuki bean epicotyl. by immunohistochemical and immunocytochemical assays.
Monoclonal Anti-β-Tubulin-FITC antibody has been used:
- in the evaluation of the status of the meiotic spindle and the arrangement of chromosomes
- in immunofluorescence studies
- in immunofluorescence microscopy
- in spindle and chromosome imagery(6)
Biochem/physiol Actions
Mutation in β-Tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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