G3153

β-Galactosidase from Escherichia coli

lyophilized powder, ≥500 units/mg protein

• Synonym(s): β-D-Galactoside galactohydrolase, Lactase
• CAS Number: 9031-11-2
• EC Number: 3.2.1.23 (BRENDA, IUBMB)
• EC Number: 232-864-1
• MDL number: MFCD00130623
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• mol wt: 465 kDa
• storage temp.: −20°C

Description

General description

β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.

Application

β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).

β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.

Packaging

Package size based on protein content

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Physical form

Stabilized with phosphate buffer and sucrose

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves