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G4256

Sigma-Aldrich

Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast

Type IV, lyophilized powder, 20-60 units/mg protein (modified Warburg-Christian)

Synonym(s):

GALT, UDP glucose:α-D-galactose-1-phosphate uridyltransferase

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type IV

Quality Level

form

lyophilized powder

specific activity

20-60 units/mg protein (modified Warburg-Christian)

composition

Protein, 15-35%

foreign activity

6-phosphogluconate dehydrogenase ≤0.5%
UDP glucose pyrophosphorylase and galactokinase ≤0.2%

storage temp.

−20°C

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General description

Research area: Cell Signaling

Galactose-1-phosphate uridyltransferase (GALT) is a galactose metabolizing enzyme that facilitates the simultaneous conversion of uridine diphosphoglucose (UDP-glucose) and galactose-1-phosphate (gal-1P) to uridine diphosphogalactose (UDP-galactose) and glucose-1-phosphate. Classic Galactosemia (CG) is an inherited metabolic condition caused by deficiency of GALT activity. GALT gene is mapped to human chromosome 9p13. Deficiency of GALT results in type 1 galactosemia.

Application

Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast has been used to perform enzyme assays.

Unit Definition

One unit will form 1.0 μmole of glucose 1-phosphate from UDP-glucose, galactose 1-phosphate and NADP+ per min at pH 8.7 at 25 °C as detected by a coupled system using phosphoglucomutase.

Physical form

Contains buffer salts as citrate and reduced glutathione

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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