G4256

Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast

Type IV, lyophilized powder, 20-60 units/mg protein (modified Warburg-Christian)

• Synonym(s): GALT, UDP glucose:α-D-galactose-1-phosphate uridyltransferase
• CAS Number: 9016-11-9
• EC Number: 2.7.7.12 (BRENDA, IUBMB)
• MDL number: MFCD00131120
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type IV
• Quality Level: 300
• form: lyophilized powder
• specific activity: 20-60 units/mg protein (modified Warburg-Christian)
• composition: Protein, 15-35%
• foreign activity: 6-phosphogluconate dehydrogenase ≤0.5%; UDP glucose pyrophosphorylase and galactokinase ≤0.2%
• storage temp.: −20°C

Description

General description

Research area: Cell Signaling

Galactose-1-phosphate uridyltransferase (GALT) is a galactose metabolizing enzyme that facilitates the simultaneous conversion of uridine diphosphoglucose (UDP-glucose) and galactose-1-phosphate (gal-1P) to uridine diphosphogalactose (UDP-galactose) and glucose-1-phosphate. Classic Galactosemia (CG) is an inherited metabolic condition caused by deficiency of GALT activity. GALT gene is mapped to human chromosome 9p13. Deficiency of GALT results in type 1 galactosemia.

Application

Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast has been used to perform enzyme assays.

Unit Definition

One unit will form 1.0 μmole of glucose 1-phosphate from UDP-glucose, galactose 1-phosphate and NADP⁺ per min at pH 8.7 at 25 °C as detected by a coupled system using phosphoglucomutase.

Physical form

Contains buffer salts as citrate and reduced glutathione

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves