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Key Documents

GLN1

Sigma-Aldrich

Glutamine and Glutamate Determination Kit

Synonym(s):

L-glutamine enzymatic quantification kit

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

usage

 kit sufficient for 50 tests

Quality Level

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

UV/Vis spectroscopy: suitable

λmax

340 nm

application(s)

cell analysis
detection

storage temp.

2-8°C

Application

For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.
Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:
  • in minimum essential medium (MEM) medium
  • in the supernatant
  • in plasma

Biochem/physiol Actions

The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD+ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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