H2625

Hemoglobin from bovine blood

suitable for protease substrate, substrate powder

• Synonym(s): Bovine hemoglobin, Hb, Methemoglobin
• CAS Number: 9008-02-0
• MDL number: MFCD00131282
• UNSPSC Code: 12352202
• eCl@ss: 42030116
• NACRES: NA.61

Properties

• biological source: bovine blood
• form: substrate powder
• mol wt: M_r ~64500
• technique(s): activity assay: suitable
• solubility: H₂O: soluble 20 mg/mL
• suitability: suitable for protease substrate
• UniProt accession no.: P01966; P02070; P02081; P06642; P06643
• storage temp.: 2-8°C
• Gene Information: cow ... HBA1(281221), HBB(280813), HBE2(513240), HBE4(513108), HBG(511735), alpha globin(512439)

Description

General description

Hemoglobin is the major component of red blood cells and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is a globular protein with α and β chains with every 141 and 146 amino acids respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.

Application

Hemoglobin from bovine blood has been used as a substrate in cathepsin D activity assay. It has also been used to measure the activity of acid proteases (pepsin-like) in the stomach extract.

The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.

Biochem/physiol Actions

Bovine hemoglobin is used in the production of hemoglobin-vesicles (HbV). It has high thermal stability and high oxygen affinity when compared to human hemoglobin. Bovine hemoglobin interacts with synthetic and azo dyes. Polymerized forms of bovine hemoglobin are used to treat autoimmune hemolytic anemia.

Oxygen transporter, NO scavenger

Oxygen transporter. The Fe²⁺/Fe³⁺ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.

Caution

Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.

Preparation Note

Prepared from washed, lysed and dialyzed erythrocytes.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves