L6150

Lysine Oxidase from Trichoderma viride

lyophilized powder, ≥20 units/mg protein

• Synonym(s): L-Lysine:oxygen oxidoreductase (deaminating)
• CAS Number: 70132-14-8
• EC Number: 1.4.3.14 (BRENDA, IUBMB)
• MDL number: MFCD00131554
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: fungus (Trichoderma viride)
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥20 units/mg protein
• mol wt: 112 kDa
• composition: Protein, 5-20%
• storage temp.: 2-8°C

Description

General description

Lysine Oxidase from Trichoderma viride is a homodimeric flavoenzyme corresponding to molecular mass of 112 kDa. It is stable at 65°C and is highly specific for L-lysine substrate. It comprises FAD-binding, substrate binding and a helical domain with distinct active site funnel.

Application

Lysine Oxidase from Trichoderma viride has been used in the preparation of luminescent biochip preparation.

Biochem/physiol Actions

Lysine Oxidase from Trichoderma viride catalyzes the formation of α-keto- ε-aminocaproate by the oxidative deamination of L-lysine. It displays anti-tumor functionality in cancer leukaemic cells. It is a tumor suppressor for squamous cell, fibroblast, ovarian and gastric tumors. Lysine oxidase also plays key role in connective tissue structural integrity and embryo development.

Unit Definition

One unit will catalyze the formation of 1 μmole of 6-amino-2-oxohexanoic acid from L-lysine per min at 37°C at pH 8.0.

Physical form

Contains phosphate buffer salts and stabilizer

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves