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LY0100

Sigma-Aldrich

Lysozyme Activity Kit

sufficient for 100 tests

Synonym(s):

Lysozyme assay kit

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26
Pricing and availability is not currently available.
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usage

sufficient for 100 tests

Quality Level

200

storage temp.

2-8°C

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assay

97%

assay

96%

assay

95%

assay

97%

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

powder

form

powder

form

solid

form

powder

mp

202-207 °C

mp

235-239 °C

mp

>300 °C

mp

219-224 °C

optical activity

[α]22/D -84.0°, c = 1 in H2O

optical activity

[α]22/D +11°, c = 1 in H2O

optical activity

[α]20/D −129°, c = 1 in H2O

optical activity

[α]22/D +258.0°, c = 1 in H2O

functional group

amine

functional group

amine

functional group

amine

functional group

amine

General description

The Lysozyme Detection Kit provides ready-to-use reagents for detecting the presence of lysozyme activity. This simple assay to detect lysozyme activity uses Micrococcus lysodeikticus cells as the substrate. Lysozyme activity results in the lysis of the Micrococcus lysodeikticus cells. During incubation of the lysozyme sample and substrate, the reaction is followed by monitoring the decrease in absorbance at 450 nm.

Application

Lysozyme Activity Kit has been used to measure the catalytic activity of native lysozyme[1], lysozyme purified from chicken egg white[2] and lysozyme from pilocarpine-stimulated saliva.[3]

Biochem/physiol Actions

Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.[4]

Storage and Stability

The Reaction Buffer is stable for at least 2 years at 2–8 °C.
The Lysozyme (Catalog Number L6876) may be stored long term at –20 °C and remains active for 4 years.
The Micrococcus lysodeikticus cells (Catalog Number M3770) should be stored long term at –20 °C.

Kit Components Also Available Separately

Product No.
Description
SDS
  • L6876Lysozyme from chicken egg white, powder or granules, ≥39,000 units/mg proteinSDS
  • Lysozyme Reaction Buffer
  • M3770Micrococcus lysodeikticus ATCC No. 4698, suitable for substrate for the assay of lysozyme, lyophilized cellsSDS

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCM1127
    SLCL8002
    SLCK7507
    SLCH6579
    SLCG6566

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    Clemens Simbrunner et al.
    ACS nano, 6(6), 4629-4638 (2012-05-19)
    We report on the epitaxial growth of periodic para-hexaphenyl (p-6P)/α-sexi-thiophene (6T) multilayer heterostructures on top of p-6P nanotemplates. By the chosen approach, 6T molecules are forced to align parallel to the p-6P template molecules, which yields highly polarized photoluminescence (PL)-emission
    Woon-Seok Kang et al.
    Korean journal of anesthesiology, 61(3), 210-215 (2011-10-26)
    Atrial fibrillation (AF) is the most common cardiac arrhythmia. Magnesium has been reported to be effective in reducing the incidence or prophylaxis of AF. Magnesium is also an essential constituent of many enzyme systems and plays a physiological role in
    Annealing effect in the sexithiophene:C70 small molecule bulk heterojunction organic photovoltaic cells
    Sakai, J.; et al.
    Solar Energy Mat. and Solar Cells, 93, 1149?1153-1149?1153 (2009)
    Clemens Simbrunner et al.
    Journal of the American Chemical Society, 133(9), 3056-3062 (2011-02-12)
    During the last years, self-assembled organic nanostructures have been recognized as a proper fundament for several electrical and optical applications. In particular, phenylenes deposited on muscovite mica have turned out to be an outstanding material combination. They tend to align
    Günther Schwabegger et al.
    Crystal growth & design, 13(2), 536-542 (2013-02-16)
    The morphology and structure of sexithiophene deposited on KCl (100) substrates was investigated by scanning force microscopy and specular X-ray diffraction measurements. Two different needle-like structures with {010} and {4̅11} contact planes have been observed as well as islands of
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