M3168

4-Methylumbelliferyl phosphate

alkaline phosphatase substrate, liquid

• Synonym(s): 4-methylumbelliferone phosphate Liquid Substrate System
• CAS Number: 3368-04-5
• EC Number: 222-137-7
• MDL number: MFCD00016969
• UNSPSC Code: 12352204
• PubChem Substance ID: 24896808
• NACRES: NA.32

Properties

• Product Name: 4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, liquid
• form: liquid
• Quality Level: 200
• storage temp.: 2-8°C
• SMILES string: CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12
• InChI: 1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
• InChI key: BCHIXGBGRHLSBE-UHFFFAOYSA-N

Description

General description

4-Methylumbelliferyl phosphate (4-MUP) is a substrate for alkaline phosphatase which forms the soluble fluorescent substance methylumbelliferone. 4-MUP Liquid Substrate System combines 4-MUP and buffer in a ready-to-use reagent.

Application

4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System has been used as a fluorescent substrate for alkaline phosphate in enzyme linked immunosorbent assay (ELISA). It has also been used in secreted embryonic alkaline phosphatase (SEAP) activity assay.

Analysis Note

4-MUP fluorescence can be measured with excitation at 360 nm and emission at 440 nm. The fluorescent end product may also be observed using a UV light source.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable