M9309

McCoy′s 5A Medium

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): 5A basal medium
• MDL number: MFCD00217560
• UNSPSC Code: 12352207
• NACRES: NA.71

Properties

• Product Name: McCoy′s 5A Medium, Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: NaHCO₃: 2.2 g/L; L-glutamine: 0.21 g/L; phenol red: 0.011 g/L; glucose: 3.0 g/L (Dextro)
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.

Application

McCoy′s 5A Medium has been used:

• to culture HCT-116?cells
• to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
• to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies

Biochem/physiol Actions

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable