N7885

Neuraminidase from Vibrio cholerae

Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)

• Synonym(s): Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase
• CAS Number: 9001-67-6
• EC Number: 3.2.1.18 (BRENDA, IUBMB)
• EC Number: 232-624-6
• MDL number: MFCD00131711
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• Quality Level: 300
• type: Type III
• form: buffered aqueous solution
• specific activity: 1-5 units/mg protein (Lowry, using NAN-lactose)
• mol wt: 83 kDa
• application(s): diagnostic assay manufacturing
• foreign activity: Protease and NAN-aldolase, present
• storage temp.: 2-8°C

Description

General description

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase.

Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Biochem/physiol Actions

Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells.

Quality

Preservative free.

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Physical form

Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl₂.

Preparation Note

Chromatographically purified.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves