N9284

Nitroreductase from Escherichia coli

≥90% (SDS-PAGE), recombinant, expressed in E. coli

• Synonym(s): NTRA
• MDL number: MFCD03457727
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥90% (SDS-PAGE)
• form: lyophilized powder
• specific activity: ≥100 units/mL
• mol wt: monomer 24000
• greener alternative product characteristics: Waste Prevention; Safer Solvents and Auxiliaries; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• UniProt accession no.: P38489
• greener alternative category: , Re-engineered
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: Escherichia coli K12 ... nfsB(945483)

Description

General description

Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.

Application

Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.

Biochem/physiol Actions

Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.

Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.

Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.

Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

Physical properties

Lyophilized powder containing PBS. Does not contain BSA as excipient

Unit Definition

One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.

Preparation Note

Produced using animal component-free materials.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable