P2165

Protein A–Biotin from Staphylococcus aureus

lyophilized powder

• MDL number: MFCD00132098
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• conjugate: biotin conjugate
• Quality Level: 200; 300
• form: lyophilized powder
• composition: Protein, ≥70% biuret
• extent of labeling: 3.0-8.0 mol biotin per mol Protein A
• storage temp.: 2-8°C

Description

General description

Protein A (SpA) is a 42kDa virulence factor produced by Staphylococcus aureus, and is composed of five highly homologous consecutive extracellular Ig-binding domains, namely domains E, D, A, B, and C. This protein is present in both secreted and membrane-associated forms.

Specificity

Binds IgG only from most mammals, except rat, goat, and sheep

Biochem/physiol Actions

Protein A (SpA) causes B cells death in vitro, by binding to the Fc region of antibody and the Fab regions of the B-cell receptor, thus inhibiting its opsonophagocytosis. This protein is thought to be implicated in the evasion of host immune responses by Staphylococcus aureus, and thus, might be a candidate for vaccine development approaches to prevent severe S. aureus infections. S. aureus is responsible for nosocomial infections as well as a variety of human infections, such as food poisoning, toxic shock syndrome, endocarditis, septicemia, skin infections, soft tissue infections, and bone infections. It is also responsible for bovine and ovine mastitis. SpA typing or DNA sequence analysis of the variable repeat region of protein A gene is useful in determining S. aureus outbreak strains from those strains that are epidemiologically insignificant.

Physical form

Lyophilized powder containing sodium citrate

Preparation Note

Prepared from P 6031, coupled to biotin by an amide bond through an aminocaproyl spacer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.