P3287

Protein kinase CβII isozyme human

>85% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

• Synonym(s): Ca²⁺-activated phospholipid-dependent serine-threonine kinase βII isozyme human, PKC βII human
• EC Number: 2.7.1.37 (BRENDA, IUBMB)
• MDL number: MFCD03458219
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected insect cells
• Assay: >85% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: ≥500 unit/mg solid
• mol wt: 80 kDa by SDS-PAGE; calculated mol wt 76.9 kDa
• UniProt accession no.: P05771
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PRKCB(5579)

Description

Biochem/physiol Actions

PKCβII is involved in glucose signaling pathways.

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP₂) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca²⁺ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca²⁺-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

Unit Definition

One unit will transfer 1 nmol of phosphate to histone H3 in 1 min at pH 7.4 at 30 °C.

Physical form

Solution in 20 mM HEPES, pH 7.4; 2 mM EDTA, 2 mM EGTA, 5 mM DTT, 100 mM NaCl, 0.05% Triton X-100, and 50% glycerol.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves