P3627

Phosphatase, Acid from wheat germ

≥0.4 unit/mg solid

• Synonym(s): (Orthophosphoric-monoester phosphohydrolase [acid optimum] ), Acid Phosphatase from wheat germ
• CAS Number: 9001-77-8
• EC Number: 3.1.3.2 (BRENDA, IUBMB)
• EC Number: 232-630-9
• MDL number: MFCD00131847
• UNSPSC Code: 12352204
• eCl@ss: 42010102
• NACRES: NA.54

Properties

• form: powder
• Quality Level: 300
• specific activity: ≥0.4 unit/mg solid
• mol wt: 58 kDa by gel filtration
• storage temp.: −20°C
• SMILES string: O(CC(O)C)CC#C
• InChI: 1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3
• InChI key: GZCWLCBFPRFLKL-UHFFFAOYSA-N

Description

Application

Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Product P3627 is from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining.

Biochem/physiol Actions

Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.

Quality

Contains lipase

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

Preparation Note

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves