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Key Documents

P7488

Sigma-Aldrich

Protein A−Alkaline Phosphatase

protein A from Staphylococcus aureus

Synonym(s):

Protein A−AP

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

protein A from Staphylococcus aureus

Quality Level

conjugate

alkaline phosphatase conjugate

color

clear light yellow

storage temp.

2-8°C

General description

Protein A is a 42 kD cell surface protein isolated from Staphylococcus aureus. It exists in secreted as well as membrane associated forms and has high binding affinity for immunoglobulins. So, this property is useful for the labeling and purification of antibodies. Protein A−Alkaline Phosphatase can be used in immunoblotting to analyse the IgG glycosylation. Protein A−Alkaline Phosphatase has specificity for most of the mammals except goat, sheep and rat.

Application

Protein A−alkaline phosphatase may be used in ELISA for analysis of IgG glycosylation.

Physical form

Solution at approx. 1 mg protein/ml in 50 mM Tris, pH 8.0, 1.0 mM magnesium chloride, 0.1 mM ZnCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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