P8849

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution

• Synonym(s): Protease inhibitor solution
• MDL number: MFCD00677817
• UNSPSC Code: 12352200
• NACRES: NA.77
• form: DMSO solution
• solubility: water: soluble
• storage temp.: −20°C

Properties

• Product Name: Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution
• biological source: synthetic
• Quality Level: 300
• form: DMSO solution
• solubility: water: soluble
• storage temp.: −20°C

Description

General description

DMSO solution designed for use in the purification of histidine-tagged proteins.

The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Specificity

Inhibits serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

Application

The cocktail has been optimized and tested for isolation of histidine-tagged proteins, with chelating agents omitted for compatibility with IMAC applications.

It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.

Features and Benefits

Specifically formulated for histidine-tagged protein purification.

Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.

Compatibility with IMAC applications due to omission of chelating agents.

Supplied in convenient packaging options.

Components

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon

Quantity

One mL is recommended for the inhibition of proteases extracted from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells in a total volume of 100 ml.

This protease inhibitor cocktail has been optimized and tested for histidine-tagged proteins

Preparation Note

This product is supplied as a clear solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of E. coli cells or 10 g of baculovirus-infected cells.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 185.0 °F - closed cup
• Flash Point(C): 85 °C - closed cup