S100HR

Sephacryl^®

100-HR, MW range 1000-100,000 Da (globular proteins)

• Synonym(s): Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)
• CAS Number: 65546-95-4
• MDL number: MFCD00165824
• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• Quality Level: 200
• description: stability: stable to 0.2M NaOH
• form: suspension (20% aqueous ethanol)
• feature: autoclavable
• matrix: crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
• particle size: 25-75 μm (wet); 25-75 μm (wet)
• pore size: 1000-100,000 Da MW range (globular proteins)
• compatibility: mode of use size exclusion chromatography
• storage temp.: 2-8°C

Description

General description

• Supplied swollen, suspended in 20% aqueous ethanol
• Autoclavable
• Stable to 0.2N sodium hydroxide (NaOH)
• Store at refrigerated temperature (2-8 °C)

Application

For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Sephacryl^® has been used:

• to develop a novel high-throughput screening assay for sickle cell disease drug discovery
• to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine

Legal Information

Sephacryl is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 100.4 - 109.4 °F - closed cup
• Flash Point(C): 38 - 43 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves