S200HR

Sephacryl^®

200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)

• Synonym(s): Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)
• CAS Number: 65546-95-4
• MDL number: MFCD00165824
• UNSPSC Code: 12352202
• NACRES: NA.56

Properties

• Quality Level: 200
• description: stability: stable to 0.2M NaOH
• form: suspension (20% aqueous ethanol)
• feature: autoclavable
• matrix: crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
• particle size: 25-75 μm (wet)
• pore size: 1-80 kDa MW range (dextrans); 5-250 kDa MW range (globular proteins)
• compatibility: mode of use size exclusion chromatography
• storage temp.: 2-8°C

Description

General description

• Supplied swollen, suspended in 20% aqueous ethanol
• Autoclavable
• Stable to 0.2N sodium hydroxide (NaOH)
• Store at refrigerated temperature (2-8 °C)

S200HR-750ML′s updated product number is GE17-0584-01

Application

For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Sephacryl^® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl^® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl^® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.

Legal Information

Sephacryl is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 100.4 - 109.4 °F - closed cup
• Flash Point(C): 38 - 43 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves