S7651
Sphingomyelinase from Bacillus cereus
lyophilized powder, ≥100 units/mg protein
Synonym(s):
Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
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About This Item
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form
lyophilized powder
Quality Level
specific activity
≥100 units/mg protein
mol wt
36873 Da
composition
Protein, ~10% Lowry
storage temp.
2-8°C
Gene Information
Bacillus cereus ATCC 14579 ... BC0671(1203022)
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General description
Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg2+-containing metalloenzyme. Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.
Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages. Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases.
Application
Sphingomyelinase from Bacillus cereus has been used:
- in the measurement of membrane capacitance in brain slices
- for testing and confirming its silkworm-killing activity
- in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures
Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease.
Biochem/physiol Actions
Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
Unit Definition
One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.
Physical form
Lyophilized powder containing potassium phosphate buffer salts and stabilizer.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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