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Key Documents

SA20

Supelco

Starch (HK) Assay Kit

sufficient for 20 assays

Synonym(s):

Starch Quantitation Kit

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About This Item

UNSPSC Code:
12164500
NACRES:
NA.84

usage

sufficient for 20 assays

Quality Level

analyte chemical class(es)

sugars

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Application

This kit is for the quantitative, enzymatic determination of native starch in food and other materials using the amyloglucosidase-hexokinase-glucose 6-phosphate dehydrogenase enzymatic assay. The hydrolysis of starch to glucose is catalyzed by amyloglucosidase. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The increase in absorbance at 340 nm is directly proportional to the glucose concentration.

Kit Components Only

Product No.
Description

  • glucose assay reagent 20 mL

  • starch assay reagent 1 mL/vial

  • starch assay standard 5 g

related product

Product No.
Description
Pricing

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids


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