SAE0005

Glucose-6-phosphate Isomerase human

recombinant, expressed in E. coli

• Synonym(s): GPI, PGI
• EC Number: 5.3.1.9 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• Assay: ≥95% (SDS-PAGE)
• specific activity: ≥200 units/mg protein
• storage temp.: −20°C

Description

Biochem/physiol Actions

Glucose-6-phosphate isomerase (GPI) is a homodimeric enzyme ubiquitously present in most organisms. GPI catalyzes the interconversion between glucose-6-phosphate and fructose-6-hosphate, the second step of the glycolytic pathway. In mammals, Glucose-6-phosphate isomerase also acts as an autocrine motility factor (AMF), a neuroleukin, and a maturation factor. GPI deficiency is the second most common erythroenzymopathy of glycolytic enzymes after pyruvate kinase deficiency. Inherited deficiency of the enzymatic activity of GPI causes hereditary nonspherocytic hemolytic anemia (HNSHA) in humans, a severe deficiency that can be associated with hydrops fetalis (a-thalassaemia), immediate neonatal death, and neurological impairment.

Physical properties

C-terminal histidine-tagged

Unit Definition

One unit will convert 1.0 mmole of D-fructose 6-phosphate to D-glucose 6-phosphate per inute at pH 7.4 at 25 °C.

Physical form

Supplied as a solution in 50 mM Tris-HCl pH 7.5, and 50% glycerol

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable