SML2135

Biotinyl tyramide

≥97% (HPLC), powder, tyramide signal amplifier

• Synonym(s): (3aS,4S,6aR)-Hexahydro-N-[2-(4-hydroxyphenyl)ethyl]-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide, BP, Biotin-Phenol
• Empirical Formula (Hill Notation): C₁₈H₂₅N₃O₃S
• CAS Number: 41994-02-9
• Molecular Weight: 363.47
• MDL number: MFCD27953026
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Product Name: Biotinyl tyramide, ≥97% (HPLC)
• Assay: ≥97% (HPLC)
• form: powder
• color: white to beige
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: −20°C
• SMILES string: O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O
• InChI: 1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1
• InChI key: VZWXNOBHWODXCW-ZOBUZTSGSA-N

Description

Application

Biotinyl tyramide or Biotin-Phenol has been used in proximity-dependent labeling for protein identification.

Biochem/physiol Actions

Biotinyl tyramide is a reagent used for tyramide signal amplification for both immunohistochemistry (IHC) and in situ hybridization protocols and with either chromogenic or fluorescence detection. Preliminary binding of a probe is followed by secondary detection of the probe with an HRP-labeled antibody or streptavidin conjugate. Catalysis by the HRP results in the deposition of multiple biotinyl tyramide molecules in the immediate vicinity of the probe that can then be detected with a labeled streptavidin conjugate. Detection sensitivity can be 100-fold or more sensitive compared to conventional detection procedures.

Reagent used for tyramide signal amplification

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3