SML2236

L-012 sodium salt

≥98% (HPLC), powder, neurotoxin

• Synonym(s): 8-Amino-5-chloro-2,3-dihydro-7-phenyl-Pyrido[3,4-d]pyridazine-1,4-dione, sodium salt, 8-Amino-5-chloro-7-phenyl-2,3-dihydro-pyrido[3,4-d]pyridazine-1,4-dione, sodium salt, L012
• Empirical Formula (Hill Notation): C₁₃H₈ClN₄O₂ · Na
• CAS Number: 143556-24-5
• Molecular Weight: 310.67
• MDL number: MFCD30378369
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Product Name: L-012 sodium salt, ≥98% (HPLC)
• Assay: ≥98% (HPLC)
• form: powder
• color: yellow to orange
• solubility: H₂O: 2 mg/mL, clear
• storage temp.: −20°C
• SMILES string: [Na+].Clc1nc(c(c3c1c(n[nH][c]3=O)[O-])N)c2ccccc2
• InChI: 1S/C13H9ClN4O2.Na/c14-11-8-7(12(19)17-18-13(8)20)9(15)10(16-11)6-4-2-1-3-5-6;/h1-5H,15H2,(H,17,19)(H,18,20);/q;+1/p-1
• InChI key: IGEUYSJHQQCEFP-UHFFFAOYSA-M

Description

Application

L-012 has been used as a probe in a bioluminescent assay for the determination of the reactive oxygen species (ROS) levels in the isolated mouse lungs.

Biochem/physiol Actions

L-012 is a luminol analog and a widely used reactive oxygen and nitrogen species (RONS; ROS & RNS) chemiluminescence (CL) probe both in cultures and in animals in vivo. L-012 displays significantly higher CL yield and sensitivity than luminol, lucigenin and MCLA. Instead of reacting with superoxide anion (O₂⁻) directly, L-012 (LumH2) is converted via a one-electron oxidation (catalyzed by peroxidase in the presence of H₂O₂) to the LumH· radical form, which then reacts with oxygen (O₂) to yield O₂⁻ and L-012 quinone (q-Lum). The O₂⁻ in turn reacts with the LumH· radical, leading eventually to an endoperoxide that decomposes to emit luminescence. To a less extend, H₂O₂ can also react with q-Lum to yield luminescence. Although not specific to NADPH oxidase-derived RONS, L-012 is also commonly used for screening NADPH oxidase inhibitors.

Widely used reactive oxygen and nitrogen species (RONS; ROS and RNS) chemiluminescence (CL) probe both in cultures and in animals in vivo.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable