SRP2051

HMG1, native from calf thymus

≥85% (SDS-PAGE)

• UNSPSC Code: 12352200
• NACRES: NA.26

Properties

• biological source: bovine (calf) thymus
• Assay: ≥85% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~24.9 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 900 μg/mL
• technique(s): membrane array: suitable
• color: colorless to clear
• NCBI accession no.: X12796
• UniProt accession no.: P10103
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: bovine ... HMG1(), HMGB1(282691)

Description

Biochem/physiol Actions

High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein. Mammalian HMG1 has two homologous DNA-binding domains HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction or unwinding, or by directly contacting with distinct cellular proteins. HMG-1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable