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T3526

Sigma-Aldrich

Anti-TUBA1B (alpha-tubulin ubiquitous) Antibody

rabbit polyclonal

Synonym(s):

Anti-K-ALPHA-1

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About This Item

MDL number:
MFCD00145921
UNSPSC Code:
12352203
NACRES:
NA.41

RM 276.00

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Product Name

Anti-Tubulin antibody produced in rabbit, whole antiserum

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

chicken, mouse

technique(s)

indirect immunofluorescence: 1:80 using cultured fibroblasts
western blot: 1:200 using chicken gizzard extract or mouse brain extract

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Hck human recombinant, expressed in E. coli BL21, vial of ≥200 units

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Hck human

Cyclin-Dependent Kinase 5 human recombinant, expressed in E. coli BL21, 50-100 units/μg protein

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Cyclin-Dependent Kinase 5 human

Acetyl-CoA carboxylase 2 human recombinant, expressed in Sf9 cells

A6861

Acetyl-CoA carboxylase 2 human

specific activity

≥1400 unit/μg protein

specific activity

1-2 unit/μg protein

specific activity

50-100 units/μg protein

specific activity

≥25 units/μg protein

form

solution

form

solution

form

solution

form

solution

recombinant

expressed in E. coli

recombinant

expressed in E. coli BL21

recombinant

expressed in E. coli BL21

recombinant

expressed in Sf9 cells

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

mol wt

42 kDa

mol wt

-

mol wt

-

mol wt

-

General description

Microtubules are composed of tubulin and several additional proteins designated microtubule associated proteins (MAPs). Tubulin is a heterodimer which consists of an α-chain and a β-chain, both of which have a molecular weight of approximately 55,000. Microtubules form an elaborate cytoplasmic network during interphase and are reorganized into the mitotic spindle during cell division.
Tubulin is an extremely conserved and negatively charged protein. It is expressed in all eukaryotic cells, but mainly in brains.

Specificity

The product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting.

Immunogen

microtubule proteins from brains of 13 day old chicken embryos

Application

Anti-Tubulin antibody produced in rabbit has been used in immunoblotting and indirect immunofluorescence.

Biochem/physiol Actions

αβ-tubulin heterodimers helps in formation of microtubules. Tubulin dimers exhibits GTPase activity, assembly and disassembly properties. It facilitates the construction of the flagellar apparatus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    038M4887V
    067M4871V
    035M4770V
    094M4769V
    113M4804V

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