T4019

Trypsin−Agarose

buffered aqueous suspension, from bovine pancreas trypsin

• MDL number: MFCD00082094
• UNSPSC Code: 12352204
• eCl@ss: 42020142
• NACRES: NA.54

Properties

• biological source: bovine pancreas (trypsin)
• Quality Level: 200
• form: buffered aqueous suspension
• concentration: ≥15 units/mL (packed gel)
• extent of labeling: ≥15 units per mL (packed gel)
• matrix: beaded agarose
• storage temp.: 2-8°C

Description

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

Physical form

Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative

Preparation Note

TPCK-treated

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable