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53346-U

Supelco

Ascentis® Express 90 Å Phenyl-Hexyl (2.7 μm) HPLC Columns

L × I.D. 15 cm × 3 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core Phenyl HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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Product Name

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 3 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L11

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

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This Item
53347-U53345-U53334-U
Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 15 cm × 3 mm

53346-U

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 3 cm × 4.6 mm

53347-U

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 10 cm × 3 mm

53345-U

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column 2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

53334-U

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

matrix active group

phenylhexyl phase

matrix active group

phenylhexyl phase

matrix active group

phenylhexyl phase

matrix active group

phenylhexyl phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

particle size

2.7 μm

particle size

2.7 μm

particle size

2.7 μm

particle size

2.7 μm

L × I.D.

15 cm × 3 mm

L × I.D.

3 cm × 4.6 mm

L × I.D.

10 cm × 3 mm

L × I.D.

5 cm × 2.1 mm

agency

suitable for USP L11

agency

suitable for USP L11

agency

suitable for USP L11

agency

suitable for USP L11

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

matrix

Fused-Core particle platform, superficially porous particle

General description

The Phenyl-Hexyl phase has unique selectivity arising from solute interaction with the aromatic ring and its delocalized electrons. It is complementary (orthogonal) to both C18 and RP-Amide phases because of this unique aromaticity. The Phenyl-Hexyl phase also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.

Application


  • A fully automated and fast method using direct sample injection combined with fused-core column on-line SPE-HPLC for determination of ochratoxin A and citrinin in lager beers.: This study presents a rapid, automated method for the detection of mycotoxins in beer using high-performance liquid chromatography. The use of the Ascentis® Express Phenyl-Hexyl column enables efficient and effective sample processing, demonstrating its applicability in food safety and quality control (Lhotska et al., 2016).

  • Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids.: This paper explores the benefits of using core-shell particle technology, including Ascentis® Express Phenyl-Hexyl columns, in the analysis of phenolic acids. The study highlights the columns′ enhanced performance and efficiency, making them valuable for chemical analysis in environmental and food samples (Chocholous et al., 2013).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    USHV001439
    USHV001438
    USHV001435
    USHV001434
    USHV001429

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    E A Carrasco-Flores et al.
    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 61(3), 509-514 (2004-12-08)
    The infrared and Raman spectra of solids and thin solid films of 6-nitrochrysene, its electronic spectra, and resonance Raman scattering (RRS) obtained with UV-laser excitation at 325 nm are reported. The vibrational assignment is supported by ab initio computations at
    Yuan-Wan Sun et al.
    Chemical research in toxicology, 22(12), 1992-1997 (2009-11-06)
    The environmental pollutant 6-nitrochrysene (6-NC) is a powerful mammary carcinogen and mutagen in rats. Our previous studies have shown that 6-NC is metabolized to trans-1,2-dihydroxy-1,2-dihydro-6-nitrochrysene (1,2-DHD-6-NC) in rats and in several in vitro systems, including human breast tissue, and the
    J V Pothuluri et al.
    Applied and environmental microbiology, 64(8), 3106-3109 (1998-08-04)
    The fungus Cunninghamella elegans was used to biotransform 6-nitrochrysene, a mutagen that is a widespread environmental contaminant. After 6 days, 74% of the 3H-labeled 6-nitrochrysene added had been metabolized to two isomeric sulfate conjugates. These conjugates were separated by high-performance
    Jacek Krzeminski et al.
    Chemical research in toxicology, 24(1), 65-72 (2010-12-01)
    Ubiquitous environmental agents [e.g., polynuclear aromatic hydrocarbons (PAHs) and their nitrated derivatives (NO(2)-PAHs)] that are known to induce mammary cancer in rodents are regarded as potential human risk factors for inducing analogous human cancers. Although 6-nitrochrysene (6-NC) is less abundant
    Wanli Xing et al.
    International journal of environmental research and public health, 17(3) (2020-02-06)
    Polycyclic aromatic hydrocarbons (PAHs) and nitro-PAHs (NPAHs) in PM2.5 samples were collected at a roadside monitoring station in Kanazawa, Japan, in every season from 2017 to 2018. Nine PAHs and five NPAHs were determined using high-performance liquid chromatography with fluorescence
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