760749

Dibenzocyclooctyne-PEG4-biotin conjugate

for Copper-free Click Chemistry

• Synonyme(s) : Polyethylene glycol, DBCO-PEG4-biotin
• Formule empirique (notation de Hill) : C₃₉H₅₁N₅O₈S
• CAS Number: 1255942-07-4
• Poids moléculaire : 749.92
• Numéro MDL: MFCD22572661
• Code UNSPSC : 12352003
• ID de substance PubChem : 329766893
• Nomenclature NACRES : NA.22

Propriétés

• Niveau de qualité: 100
• Forme: solid
• Capacité de réaction: reaction type: click chemistry
• Température de stockage: −20°C
• Chaîne SMILES: O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)NCCOCCOCCOCCOCCC(=O)NCCC(=O)N3Cc4ccccc4C#Cc5ccccc35
• InChI: 1S/C39H51N5O8S/c45-35(12-6-5-11-34-38-32(28-53-34)42-39(48)43-38)41-18-20-50-22-24-52-26-25-51-23-21-49-19-16-36(46)40-17-15-37(47)44-27-31-9-2-1-7-29(31)13-14-30-8-3-4-10-33(30)44/h1-4,7-10,32,34,38H,5-6,11-12,15-28H2,(H,40,46)(H,41,45)(H2,42,43,48)/t32-,34-,38-/m0/s1
• Clé InChI: LNHSQAOQVNHUGL-QRBHCBQLSA-N

Description

Description générale

This azadibenzocyclooctyne-biotin derivative is a versatile biotinylation reagent for labeling azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions.This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. Subsequent labeled molecules can be purified using streptavidin or avidin affinity reagents. The PEG spacer makes the overall compound more hydrophilic and allows for greater distance between the reaction site with the azide and the biotin detecting group.

Application

Automate your Biotin tagging with Synple Automated Synthesis Platform (SYNPLE-SC002)

Caractéristiques et avantages

More efficient labeling will occur at higher temperatures and with greater excess of biotin reagent. Typical reaction times are up to 4 hours, but longer incubation periods should be used if concentration is low or if low labeling is observed. Product is most efficient when first dissolved in a water miscible organic solvent and then diluted in aqueous reaction buffer when needed. DBCO-PEG4-Biotin is soluble in aqueous buffers up to 0.35 mM.

Autres remarques

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable