700147P

PhotoClick Cholesterol

Hex-5′-ynyl 3β-hydroxy-6-diazirinyl-5α-cholan-24-oate, powder

• Synonyme(s) : hex-5-yn-1-yl 4-((3S,5S)-3-hydroxy-10, 13-dimethyl-1,2,3,4,5,7,8,9,10,11,12,13,14,15,16,17-hexadecahydrospiro[cyclopenta[a]phenanthrene-6,3′-diazirin]-17-yl)pentanoate
• Formule empirique (notation de Hill) : C₃₀H₄₆N₂O₃
• CAS Number: 1485490-47-8
• Poids moléculaire : 482.70
• Numéro MDL: MFCD00870345
• Code UNSPSC : 12352211
• Nomenclature NACRES : NA.25

Propriétés

• Essai: >99% (TLC)
• Forme: powder
• Conditionnement: pkg of 1 × 100 μg (with stopper and crimp cap (700147P-100ug)); pkg of 1 × 250 μg (with stopper and crimp cap (700147P-250ug))
• Fabricant/nom de marque: Avanti Research^™ - A Croda Brand 700147P
• Type de lipide: click reagents
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

PhotoClick Cholesterol is a clickable, photoreactive sterol probe for use in combination with quantitative mass spectrometry to globally map cholesterol-protein interactions directly in living cells. In addition to the UV-activatable cross-linking diazirine group that reacts with proteins, the sterol contains an alkyne group for incorporation of reporter and affinity tags through click-chemistry.

PhotoClick Cholesterol is a synthetic analog of cholesterol and is photoreactive. It comprises the alkyne group.

Application

PhotoClick Cholesterol has been used:

• in lysosome-associated membrane protein 1 (LAMP1) crosslinking studies
• as an analog of cholesterol in binding and competition assay of sterol binding proteins from yeast
• as a conjugate with the ectodomain of lysosomal integral membrane protein-2 (LIMP-2)

Actions biochimiques/physiologiques

PhotoClick Cholesterol serves as a probe for tracking cholesterol-binding proteins. The copper-catalyzed azide-alkyne cycloaddition is the basis for its probe functionality. The alkyne group is essential for the click chemistry property. Its binding to partners is mediated by photocrosslinking.

Conditionnement

2 mL Amber Serum Vial with Stopper and Crimp Cap (700147P-100ug)

2 mL Amber Serum Vial with Stopper and Crimp Cap (700147P-250ug)

Informations légales

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids