04-001

Anti-JAK2 Antibody, clone 8E10.2

clone 8E10.2, Upstate^®, from mouse

• Synonyme(s) : Janus kinase 2, Tyrosine kinase
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 8E10.2, monoclonal
• Espèces réactives: mouse, human, rat
• Conditionnement: antibody small pack of 25 μg
• Fabricant/nom de marque: Upstate^®
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_004963
• Numéro d'accès UniProt: O60674
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... JAK2(3717) ; mouse ... Jak2(16452) ; rat ... Jak2(24514)

Description

Description générale

JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerizes and translocates to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.

Spécificité

This antibody recognizes Jak2.

Immunogène

KLH-conjugated linear peptide.

Application

Western Blot Analysis: 0.5-1 μg/mL of this lot was used to detect Jak2 in L6 cell lysates.

Immunohistochemistry(Paraffin):
A previous lot of this antibody was used in immunohistochemistry(paraffin) staining with eptiope retrieval: Human Cancer Array.

Anti-JAK2 Antibody, clone 8E10.2 detects level of JAK2 & has been published & validated for use in WB, IH(P).

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

Qualité

Routinely evaluated by western blot on L6 cell lysate.

Description de la cible

130 kDa

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
L6 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.