04-037

Anti-RalB Antibody, clone 25

clone 25, Upstate^®, from mouse

• Synonyme(s) : RAS-like protein B, v-ral simian leukemia viral oncogene homolog B, GTP binding protein, v-ral simian leukemia viral oncogene homolog B
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 25, monoclonal
• Espèces réactives: mouse, rat, human
• Fabricant/nom de marque: Upstate^®
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NM_002881.2
• Numéro d'accès UniProt: P11234
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RALB(5899)

Description

Description générale

RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarizatied exocystossis in epithelaial cells (Cascone, 2008). RalB is required for metastatic formation and cell survival by driving exocyst assembly during polarized cell migration (Cascone, 2008). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes

Spécificité

Other species have not been tested.

This antibody is specific for RalB and does not detect RalA.

Immunogène

Epitope: C-terminus

Synthetic peptide of the C-terminus of RalB

Application

Detect RalB using this Anti-RalB Antibody, clone 25 validated for use in WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

G-proteins

Qualité

Evaluated by western blot on HEK293 or NIH/3T3 cell lysates.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect RalB in HEK293, NIH/3T3, and rat liver cell lysate.

Description de la cible

24 kDa

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8ºC from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable