04-642

Anti-Ago2 Antibody, clone 9E8.2

ascites fluid, clone 9E8.2, Upstate^®

• Synonyme(s) : Argonaute-2, Eukaryotic translation initiation factor 2C, AGO2, eIF-2C, Slicer protein, MGC3183, Piwi domain protein, PPD
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: 9E8.2, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Upstate^®
• Technique(s): ChIP: suitable (ChIP-seq); western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NM_012154
• Numéro d'accès UniProt: Q9UKV8
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... AGO2(27161)

Description

Description générale

Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).

Spécificité

human Ago2

Immunogène

Epitope: near the N-terminus of Ago2

KLH-conjugated, synthetic peptide containing the sequence YSGAGPALAPPAPPPPIQG

Application

Anti-Ago2 Antibody, clone 9E8.2 is a Mouse Monoclonal Antibody for detection of Ago2 also known as Argonaute-2, Eukaryotic translation initiation factor 2C & has been validated in WB and has been demonstrated to perform in ChIP and ChIP-seq.

ChIP and ChIP-seq: A representative lot of this antibody was used to perform ChIP and ChIP-seq ( Woolnough, JL, Atwood, BL, and Giles KE (2015), MCB Vol. 35 No. 13, p. 2278-2294).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Chromatin Biology

RNA Binding Protein (RBP)

Qualité

Routinely evaluated by immunoblot.

Description de la cible

~100 kda

Forme physique

Ascites with 0.05% sodium azide

ImmunoAffinity Purified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable