14-864

PAK-1 PBD Protein (agarose free), 300 µg

For use in Affinity Binding Assays, ELISA & G-Protein Assays.

• Synonyme(s) : p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1
• Code UNSPSC : 12352202
• eCl@ss : 32160405
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Poids mol.: M_w 21 kDa
• Fabricant/nom de marque: Upstate^®
• Concentration: >50% (Coomassie blue staining, SDS-PAGE); 2 mg/mL
• Technique(s): ELISA: suitable; affinity binding assay: suitable (G-protein)
• Numéro d'accès NCBI: NM_002576.3
• Numéro d'accès UniProt: Q13153
• Conditions d'expédition: wet ice

Description

Description générale

Product Source: GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1, expressed in E. coli.

Qualité

Routinely evaluated by G-protein activity assay

Forme physique

300 µg of PAK-1 PBD, GST in 150 µL of PBS, pH 7.4, 0.5 mM PMSF, containing 20% glycerol.

Stockage et stabilité

Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Remarque sur l'analyse

Specific Activity: Affinity Precipitation Assay: 10 µg of this lot when added with 10 µL glutathione agarose, precipitated Rac-GTP from 3T3 and A431 cell lysates that were loaded in vitro with GTPγS. GDP treated of 3T3 and A431 lysates served as negative controls. Precipitated Rac-GTP was detected by immunoblot analysis using 1 &micor;g/mL anti-Rac (Catalog # 05-389) and secondary goat anti-mouse HRP antibody (Catalog # 12-349) on a Western Blot.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2