324725

Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli

Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins.

• Synonyme(s) : Endo-β-N-acetylglucosaminidase F1, Endo F1
• Numéro CE : 3.2.1.96 (BRENDA, IUBMB)
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 100
• Conjugué: (N-linked)
• Forme: liquid
• Activité spécifique: ≥16 units/mg protein; ≥17 units/mL
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: do not freeze
• Activité étrangère: Proteases, none detected
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Note: 1 mU = 1 milliunit.

Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5.

Forme physique

In 20 mM Tris-HCl, pH 7.5.

Autres remarques

Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable